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Of the control strain J673 (MATa ura3-52 leu2-3 leu2-112 trp163 gcn2 ) was described previously [51]. The temperature-sensitive eIF2a strain TD304-10B (MATa his4303 ura3-52 leu2-3 leu2-112 sui2-1) is a derivative of the previously described sui2-1 strain 117-8AR20 [44]. A DNA fragment encoding RCV-Z vIF2a [39] was amplified by PCR using viral DNA as a template and primers C27 (5'- TAGGATCCA

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Ere fixed in 1.5 glutaraldehyde (Serva) diluted in 0.1M cacodylate buffer, pH 7.4 stored for 20 min at room temperature, post-fixed in 1 OsO4 for 60 min at 4 , dehydrated in ethanol, and finally embedded in Dow Epoxy Resin (DER 332, Unione Chimica Europea, Milano, Italy). For double immunogold labelling ultrathin sections, cut by an ultramicrotome (Ultracut UCT; Leica, Wien, Austria) were mounte
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